The thermal denaturations of five revertant h repressors containing single amino acid substitutions in their N-terminal domains have been studied by differential scanning calorimetry. Two substitutions slightly decrease stability, and the remaining three render the protein more stable than wild type
Analysis of the effectiveness of proline substitutions and glycine replacements in increasing the stability of phage T4 lysozyme
β Scribed by H. Nicholson; D. E. Tronrud; W. J. Becktel; B. W. Matthews
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1992
- Tongue
- English
- Weight
- 905 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0006-3525
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Surface tension kinetics exhibited by the wild type and selected stability mutants of T4 lysozyme at the air-water interface were monitored with DuNouy tensiometry. Mutant lysozymes were produced by substitution of the isoleucine at position 3 with cysteine, leucine, glycine, and tryptophan. Each su
Endomorphin II (ENDII), an endogenous ligand for the mu-opioid receptor, was investigated as a possible analgesic with fewer side effects than morphine. To improve CNS entry of END II, structural modification was also examined to determine whether Pro(4) substitution and cationization affected physi
The mass fragmentation of 4-arylaminocytosioe derivatives was investigated and it was found that the ortho effect is mainly responsible for the strong stabilization of even-electron ions formed during fragmentation. The ortho effect in this class of compounds completely eliminates other possible fra