Analysis of the effect of radiation repair mutations on theDEL1mutator region ofSaccharomyces cerevisiae

✍ Scribed by K. M. Downs; A. E. Szwast; S. W. Liebman

• Publisher: Springer-Verlag
• Year: 1983
• Tongue: English
• Weight: 410 KB
• Volume: 7
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00365681

✦ Synopsis

In DELl strains of the yeast, Saccharomyces cerevisiae, the iso-l-cytochrome c (CYC1) region is flanked on either side by Tyl elements in direct orientation which promote cycl deletions of the bracketed DNA in the haploid cell. In this study, we asked which genes might control this event by testing the possibility that the DELl mutation mechanism requires an enzyme (or enzymes) that is also utilized in the repair of damaged DNA. To this end, we independently coupled eight repair mutations, rad3-2, rad4-4, rad6-1, rad6-3, rad9-1, rev3-1, rad50-1, and rad51-1, to DELl and asked whether DELl was still functional. We found that none of these rad mutations significantly affects the mutation frequency of 10-6-10 -5 established in DELl strains for the CYC1 locus. Furthermore, we determined that ste7, a temperature-sensitive sterile allele known to alter gene regulation in Ty-mediated mutations, is not required for DELl function. Finally, DELl is not temperature-sensitive at 23 ° or 37 °C.