Analysis of the Artificial Sweetener Aspartame by Means of Liquid Chromatography/Electrospray Mass Spectrometry

✍ Scribed by Guido C. Galletti; Paola Bocchini; A. M. Gioacchini; A. Roda

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 248 KB
• Volume: 10
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(19960715)10:9<1153::aid-rcm613>3.0.co;2-8

✦ Synopsis

The establishment of analytical conditions for the determination of aspartame (N-L-a-aspartyl-L-phenylalanine 1-methyl ester) by means of high-performance liquid chromatographylelectrospray mass spectrometry (HPLCI ES-MS) was studied. Aspartame was analysed in the positive-ion mode at 3.05 kV probe voltage with a methanovwater mobile phase (30:70, pH 3.01 by addition of acetic acid). Mass spectra at different spraying capillary voltages are reported and discussed. A detection limit of 5pg and a linear response for aspartame in aqueous solution in the range 1&200 pg were obtained. The method was applied to the detection of aspartame in three soft drinks commercially available in Italy.

HPLUES-MS provides an additional tool for the sensitive and selective detection of aspartame and for the improvement of possibly critical separations such as that of aspartame from d e i n e .