Polyacrylamide gel electrophoresis of fluorophorelabeled saccharides is a simple and sensitive separation method for glycan analysis. 'This method requires an aldehydic reducing carbon on the saccharide in order to react with the amine group of the fluorophore \((8-\mathrm{ami}\) nonaphthalene-1,3,6
Analysis of starch structure using fluorophore-assisted carbohydrate electrophoresis
โ Scribed by Dr. Mathew K. Morell; Michael S. Samuel; Michael G. O'Shea
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 944 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0173-0835
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We have developed a method for monitoring the \(\mathrm{N}\). glycosylation of recombinant glycoproteins directly from conditioned medium samples. Proteins in the conditioned medium are separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroblotted onto polyvinylidene fluo
## Abstract We report the use of fluorophoreโassisted carbohydrate electrophoresis (FACE) to determine the molecular mass (__M__) values of heparins (Heps) and lowโmolecularโweight (LMW)โHep derivatives. Hep are labeled with 8โaminonaphthaleneโ1,3,6โtrisulfonic acid and FACE is able to resolve each