We have developed a method for monitoring the \(\mathrm{N}\). glycosylation of recombinant glycoproteins directly from conditioned medium samples. Proteins in the conditioned medium are separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroblotted onto polyvinylidene fluo
A Gel Retardation Assay for the Interaction of Proteins and Carbohydrates by Fluorophore-Assisted Carbohydrate Electrophoresis
โ Scribed by G.F. Hu; B.L. Vallee
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 671 KB
- Volume
- 218
- Category
- Article
- ISSN
- 0003-2697
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## Abstract We report the use of fluorophoreโassisted carbohydrate electrophoresis (FACE) to determine the molecular mass (__M__) values of heparins (Heps) and lowโmolecularโweight (LMW)โHep derivatives. Hep are labeled with 8โaminonaphthaleneโ1,3,6โtrisulfonic acid and FACE is able to resolve each
A method is described for determination of carbohydrate and protein contents of glycoproteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then electroblotted onto polyvinylidene difluoride (PVDF) membranes. Blots were stained, and appropriate pieces of PVDF membranes w