Analysis of retinal and 3-dehydroretinal in the retina by high-pressure liquid chromatography

✍ Scribed by Tatsuo Suzuki; Momoyo Makino-Tasaka

• Book ID: 102986873
• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 637 KB
• Volume: 129
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(83)90059-3

✦ Synopsis

A sensitive analytical method was developed in order to study the rhodopsin-porphyropsin system in the eye. Oximes of 1 l&-retinal, all-trans-retinal, 1 I-ci+3dehydroretinaJ, and alltrans-3dehydroretinal were determined quantitatively by high-pressure liquid chromatography. This method was applied to the analysis of retinal and 3dehydroretinal in the retinas of bullfrog and goldfish. The results agreed with those obtained from the bleaching kinetics of visual pigment extracted with detergent. A reliable result is obtained if the tissue contains more than 5 pmol of retinal (or 3-dehydroretinal). The chromophore composition could be determined in the eye of a small freshwater prawn, Palaemon pancidence, using 50 pmol of I I-c&retinal and no 3dehydroretinal.

MATERIAL AND METHODS

All operations other than HPLC were done under dim red light to prevent visual pigment III