Analysis of ras gene mutations in childhood myeloid leukaemia
β Scribed by Christine Farr; Robert Gill; Fay Katz; Barbara Gibbons; Christopher J. Marshall
- Book ID
- 114712664
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 496 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0007-1048
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Mutations at codon I 2, I 3, and 6 I of the HRAS, KRAS, and NRASgenes were evaluated in 99 cases of pediatric acute myeloid leukemia (AML) using oligonucleotide hybridization to polymerase chain reacted derived products. Twenty-four mutations were identified in the NRAS gene, I 3 in the KRAS gene, a
## Abstract Nβras gene activation occurs via single base substitutions in codons 12, 13, and 61. We have developed a rapid screening method, termed allele specific restriction analysis (ASRA), for detection of Nβras mutations at these three critical codons in acute myeloid leukemia (AML). Patient D