Analysis of polyadenylatedRNAfrom human heart mitochondria
β Scribed by Michel Villalta; Julio Montoya
- Publisher
- Springer-Verlag
- Year
- 1991
- Tongue
- English
- Weight
- 237 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0172-8083
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β¦ Synopsis
The content of the mitochondrial poly(A)-RNA fraction of human heart has been analyzed by electrophoresis through agarose slab gels in the presence of methyl mercury hydroxide and staining with ethidium bromide. It is possible to identify all the heavy strand coded mRNAs in the electrophoretic pattern. This pattern is qualitatively similar to that obtained from the mitochondria of cells cultured in vitro (HeLa cells), although differences in the relative amount of some components have been detected.
π SIMILAR VOLUMES
99mTc-gluconate has previously been shown to bind to isolated rat heart mitochondria. In the presence of potassium cyanide this binding is enhanced several fold, an effect which has been shown to be correlated to the efficiency of the in vivo binding of the isotope complex in ischemic dog hearts. Th