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Analysis of pCU1 replication origins: dependence of oriS on the plasmid-encoded replication initiation protein RepA

โœ Scribed by O.P Zoueva; V.N Iyer; T.I Matula; M Kozlowski


Book ID
114122035
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
344 KB
Volume
49
Category
Article
ISSN
0147-619X

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RepAl protein is essential for replication of the RepFIC replicon of enterotoxin plasmid EntP307 and is thought to interact directly with the origin of replication. We have purified RepAl from an over-producing expression system and have prepared single crystals using a macroseeding technique. The c