✦ LIBER ✦
Crystallization and preliminary crystallographic analysis of RepA1, a replication control protein of the RepFIC replicon of enterotoxin plasmid EntP307
✍ Scribed by Haiwei Song; Simon E.V. Phillips; Mark R. Parsons; Renata Maas
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 212 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0887-3585
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✦ Synopsis
RepAl protein is essential for replication of the RepFIC replicon of enterotoxin plasmid EntP307 and is thought to interact directly with the origin of replication. We have purified RepAl from an over-producing expression system and have prepared single crystals using a macroseeding technique. The crystals belong to space group P2,2'2, or P2'2,2, with cell dimensions a = 61 A, b = 67 A, and c = 243 A. They diffract X-rays to 3.3 hi resolution and probably contain two 40,000 molecular weight RepAl molecules per asymmetric unit.