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Analysis of oligospirostanosides in a suspension culture of Dioscorea deltoidea cells by high-performance liquid chromatography

✍ Scribed by V. N. Paukov; I. S. Vasil'eva; N. N. Karasev; V. A. Paseshnichenko


Book ID
104828796
Publisher
Springer
Year
1988
Tongue
English
Weight
224 KB
Volume
24
Category
Article
ISSN
0009-3130

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An isocratic reverse-phase high-performance liquid chromatography method for the separation and quantitation of total pyridine dinucleotides in hepatocyte cultures is described. Cells are extracted with cold 3 M perchloric acid or 0.5 N sodium hydroxide containing 50% (v/v) ethanol and 35% cesium ch