Analysis of oligodendroglial differentiation using cDNA arrays

✍ Scribed by Marina Scarlato; Jacqueline Beesley; David Pleasure

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 221 KB
• Volume: 59
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/(sici)1097-4547(20000201)59:3<430::aid-jnr18>3.0.co;2-7

✦ Synopsis

We used cDNA arrays to investigate molecular aspects of the differentiation of an immortalized line of oligodendroglial progenitors, and of immunopan-purified primary cultures of oligodendroglial progenitors, to immature oligodendroglia. Developmental regulation of the proteolipid and 2-hydroxyacylsphingosine 1-galactosyltransferase genes was tighter in the primary than in the immortalized cells. Our data suggest that increased expression of genes encoding the following proteins are involved in oligodendroglial differentiation: Fyn, Erk, p85, G-␣-12 guanine nucleotide binding, and transducin ␤-2 signal transduction molecules; glial maturation factor; the proteasomal subunits C8 and C3; the proteasomal targeting molecule polyubiquitin; the cell cycle regulatory proteins Set, protein phosphatase 2A, and nuclear tyrosine phosphatase (PRL-1); and the high-affinity glutamate cotransporter EAAC-1.