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Optimization of cDNA Representational Difference Analysis for the Identification of Differentially Expressed mRNAs

✍ Scribed by Kirk Pastorian; Leo Hawel III; Craig V. Byus

Publisher: Elsevier Science
Year: 2000
Tongue: English
Weight: 221 KB
Volume: 283
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.2000.4622

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✦ Synopsis

Representational difference analysis (RDA) is a powerful and sensitive tool for identification of differentially expressed genes (M. Hubank and D. G. Schatz, 1999, Methods Enzymol. 303, 325-349; 1994, Nucleic Acids Res. 22, 5640 -5648) that will identify both up-and downregulated genes differentially expressed between two cDNA populations. This manuscript provides a thorough description of an optimized RDA method. This procedure while still based on the traditional RDA originally developed by Lisitsyn and coworkers(N.

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