Repeated DNA sequences of Neurospora crassa were isolated and characterized. Approximately 10-12% of N. crassa DNA sequence were repeated, of which 7.3% were found to be transcribed in mid-log phase of mycelial growth as measured by DNA:RNA hybridization. It is suggested that part of repetitive DNA
Analysis of nutrient-dependent transcript variations in Neurospora crassa
✍ Scribed by Verena Aign; Jörg D Hoheisel
- Book ID
- 117730677
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 629 KB
- Volume
- 40
- Category
- Article
- ISSN
- 1087-1845
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Transcriptional properties of an "albino" mutant, a "slime" mutant and a wild type strain of Neurospora crassa have been compared by quantitative estimates of DNA:RNA hybridization. The RNAs isolated from these strains were incubated with 32P-DNA of the wild type strain. The DNA:RNA hybrids were iso
Mycelia of Neurospora crassa growing with quite different rates at a given temperature (30 ~ C) are obtained by changing the composition of the culture medium: for instance a duplication time of 220 min is found when cultures are growing in Vogel's minimal medium supplemented with glycerol and one o