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Analysis of MTF degradation in the imaging of cells in a flow system

✍ Scribed by S. H. Ong; P. M. Nickolls


Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
603 KB
Volume
5
Category
Article
ISSN
0899-9457

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✦ Synopsis


Abstract

The time‐delay and integration method can be used with a charge‐coupled device array to acquire images of cell in a flow cytometer. The loss of high spatial frequency components in images acquired with this technique is studied by deriving the modulation transfer function (MTF) for each of the main causes of image degradation: finite sampling aperture, nonsynchronism between image movement and charge transfer rate, discrete charge motion, transfer inefficiency, and axis misalignment. The system MTF and resolution in the horizontal and vertical directions are then examined.Β©1994 John Wiley & Sons Inc


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