Analysis of MTF degradation in the imaging of cells in a flow system
β Scribed by S. H. Ong; P. M. Nickolls
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 603 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0899-9457
No coin nor oath required. For personal study only.
β¦ Synopsis
Abstract
The timeβdelay and integration method can be used with a chargeβcoupled device array to acquire images of cell in a flow cytometer. The loss of high spatial frequency components in images acquired with this technique is studied by deriving the modulation transfer function (MTF) for each of the main causes of image degradation: finite sampling aperture, nonsynchronism between image movement and charge transfer rate, discrete charge motion, transfer inefficiency, and axis misalignment. The system MTF and resolution in the horizontal and vertical directions are then examined.Β©1994 John Wiley & Sons Inc
π SIMILAR VOLUMES
## Abstract The effects on the phase of spins moving during echoβplanar imaging (EPI) acquisition were studied. Standard singleβshot and interleaved multishot blipped EPI acquisitions were considered, assuming either high gradient strength and slew rates or standard gradient strength and slew rates
A progjam for automated control of cell enumeration, microscope stage and focus adjustment and statistical analysis of data is described. The program is designed to run on an Hewlett-Packard 9835A desktop computer programmable in Hewlett-Packard Enhanced BASIC.
Plant-cultured cells are often highly heterogeneous in secondary metabolite productivity. The industrial application for large-scale metabolite production requires establishment of a stable high-producing cell line. In this study, image analysis of the individual cell is investigated as a method for