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Analysis of pigment accumulation heterogeneity in plant cell population by image-processing system

✍ Scribed by Kazuhiko Miyanaga; Minoru Seki; Shintaro Furusaki

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 126 KB
Volume: 67
Category: Article
ISSN: 0006-3592
DOI: 10.1002/(sici)1097-0290(20000220)67:4<493::aid-bit13>3.0.co;2-u

No coin nor oath required. For personal study only.

✦ Synopsis

Plant-cultured cells are often highly heterogeneous in secondary metabolite productivity. The industrial application for large-scale metabolite production requires establishment of a stable high-producing cell line. In this study, image analysis of the individual cell is investigated as a method for evaluation of a heterogeneous cell population, and compared with the conventional method of estimation, which is based on averagecell productivity. Among strawberry cells producing anthocyanins, cells with a wide-range of pigment concentration were observed and maximum anthocyanin content was 10 times higher than the average value. In addition, a change of the frequency distribution was revealed in batch cultivation.

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