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Analysis of mono-, di- and oligosaccharides by CE using a two-stage derivatization method and LIF detection

โœ Scribed by Hua-Ming Tseng; Stefano Gattolin; Jeremy Pritchard; H. John Newbury; David A. Barrett


Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
146 KB
Volume
30
Category
Article
ISSN
0173-0835

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Monosaccharides, disaccharides and larger carbohydrates can be derivatized using 3-aminophenylboronic acid (3-APBA). This procedure is carried out at low pH (2.7-3.0) and allows the use of positive ion mode electrospray orthogonal time-of-flight mass spectrometry (ES-OTOFMS) to analyze the resulting