Oligosaccharide profiling: the facile detection of mono-, di- and oligosaccharides by electrospray orthogonal time-of-flight mass spectrometry using 3-aminophenylboronic acid derivatization
✍ Scribed by Dudley Williams; Terry D. Lee; Nikita Dinh; Mary K. Young
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 111 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0951-4198
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✦ Synopsis
Monosaccharides, disaccharides and larger carbohydrates can be derivatized using 3-aminophenylboronic acid (3-APBA). This procedure is carried out at low pH (2.7-3.0) and allows the use of positive ion mode electrospray orthogonal time-of-flight mass spectrometry (ES-OTOFMS) to analyze the resulting boronate complexes. A carbohydrate profile map of a complex carbohydrate mixture, honey, was prepared which displayed superior sensitivity when compared with lithium ion cationization. Complexes formed using simple mono-and disaccharides show that facile in situ derivatization leads to an equilibrium mixture; which is reproducible for a specific set of electrospray conditions. D-Glucose could be detected at 5 mM concentration using the standard instrument spray interface. Lower detection levels of approximately 500 nM could be achieved using a nanospray device. The 3-APBA complexes are observed on instruments employing a low temperature interface (140-150 °C) which allows formation of the boronate species while still promoting efficient desolvation of the ions. The spectral identification of 3-APBA complexed carbohydrates in complex mixtures is facilitated by the easily observed 1 mass unit separated peak pair bearing the 1:4 ratio resulting from the natural isotopic abundance of 10 B and 11 B.