Analysis of microcystins by capillary high performance liquid chromatography using a polymethacrylate-based monolithic column

✍ Scribed by Congying Gu; Li Lin; Xiaodong Chen; Jinping Jia; Dan Wu; Nenghu Fang

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 945 KB
• Volume: 30
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200700277

✦ Synopsis

Abstract

A polymethacrylate‐based monolithic column was prepared and its application to the separation of three kinds of similar microcystins (MCs) in capillary high performance liquid chromatography (capillary‐HPLC) with ultraviolet detection was studied. The monolithic matrix contains both hydrophobic and cation‐exchange interaction sites. Factors influencing the separation performance have been investigated. A baseline separation could be achieved by means of Tris(hydroxymethyl)aminomethane buffer of mildly alkaline pH and acetonitrile as the mobile phases in less than 5 min. The calibration curves were linear with a correlation coefficient r >0.9990 over a range of 0.25–18.00 mg/L. This method was successfully applied to the separation of microcystins from other compounds in spiked uncontaminated lake water after performing solid‐phase extraction. The whole procedure provided low LODs, e. g. the LODs for MC‐LR, MC‐YR, and MC‐RR were found to be 0.49, 0.67, 0.30 μg/L, respectively. The LODs, precision, efficiency, and the results obtained for the real samples demonstrate the potential of polymethacrylate‐based monolithic columns as fast separation tools for routine use in the monitoring of microcystins in real water samples.