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Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome and peptide profiling analysis by using a monolithic analytical capillary column

✍ Scribed by Xiaogang Jiang; Jing Dong; Fangjun Wang; Shun Feng; Mingliang Ye; Hanfa Zou


Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
289 KB
Volume
29
Category
Article
ISSN
0173-0835

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An on-line method has been developed for the derivatization and coupled liquid chromatography (LC)/electrospray ionization (ESI) MS analysis of peptides at the femtomol level. Peptides are reacted with N-succinimidyl-2(3-pyridyl)acetate (SPA) in buffered aqueous medium at pH 7 following loading on a