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Analysis of Ligase Chain Reaction Products via Matrix-Assisted Laser Desorption/Ionization Time-of-Flight–Mass Spectrometry

✍ Scribed by Christian Jurinke; Dirk van den Boom; Anette Jacob; Kai Tang; Ralf Wörl; Hubert Köster

Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
132 KB
Volume
237
Category
Article
ISSN
0003-2697

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✦ Synopsis

powerful tools. Commonly used DNA-based techniques A rapid and accurate detection of ligation products for this purpose are restriction fragment-length polygenerated in ligase chain reactions (LCR) by using morphism, single-strand conformation polymorphism, matrix-assisted laser desorption/ionization time-ofand the use of allele-specific oligonucleotides. The liflight-mass spectrometry (MALDI-TOF-MS) is regase chain reaction (LCR) 2 represents another DNAported. LCR with Pfu DNA ligase was performed with based technology with a high potential for diagnostic a wild-type template and a template carrying a single applications (5).

HPLC, ion-exchange high-pressure liquid chromatography; LCR, ligase chain reaction; MALDI-TOF-MS, matrix-assisted laser desorption/ionization time-of-flight-mass spectrometry; oligo, oligonu-

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