Analysis of intranuclear binding process of glucocorticoid receptor using fluorescence correlation spectroscopy
β Scribed by Shintaro Mikuni; Mamoru Tamura; Masataka Kinjo
- Book ID
- 118442159
- Publisher
- Elsevier Science
- Year
- 2007
- Tongue
- English
- Weight
- 447 KB
- Volume
- 581
- Category
- Article
- ISSN
- 0014-5793
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Fluorescence correlation spectroscopy (FCS) is a well-established method for the analysis of freely diffusing fluorescent particles in solution. In a two-colour setup, simultaneous detection of two different dyes allows the acquisition of both the autocorrelation of the signal of each channel and th
Receptor binding studies with the conventional method require the use of radioactively labelled ligands and include several washing steps. In certain cases, the halflife of the receptor-ligand complex is often shorter or similar to the time required for separation. Interactions between certain ligan
Fluorescence correlation spectroscopy (FCS) provides information about translational diffusion properties of fluorescent molecules in tiny detection volume and allows the analysis of binding processes of biomolecules in homogeneous solution. In this study, FCS was used to measure equilibrium binding