## Abstract The DNA of tumor tissue K1 obtained at autopsy from a case of hepatocellular carcinoma (HCC) in a 9βyearβold boy contained integrated hepatitis B virus (HBV) DNA at a single site in the chromosome (case 2, Chang et al.: Hepatology 13:316β320, 1991). To characterize further the integrate
Analysis of integrated hepatitis B virus DNA and flanking cellular sequence by inverse polymerase chain reaction
β Scribed by Po-Chin Wang; Eric Ka-Wai Hui; Jen-Hwey Chiu; Szecheng J Lo
- Book ID
- 114139604
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 134 KB
- Volume
- 92
- Category
- Article
- ISSN
- 0166-0934
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By digestion with Hindlll restriction enzyme, a human hepatocellular carcinoma was shown to contain only 2 hepatitis B virus (HBV) DNA inserts. Both Hindlll fragments (8 and 16 kb) were molecularly cloned and the structures of HBV DNA and adjacent host sequences were analyzed. One clone (AYH 8) cont
## Abstract Hepatitis B virus (HBV) DNA integration into host chromosomes is detected in more than 80% of HBVβrelated hepatocellular carcinomas (HCC), yet its significance in tumor development remains obscure. In this study, we reβexamined the integration pattern of HBV in childhood HCC tissues, wh
The polymerase chain reaction (PCR) technique has been utilized for the detection of hepatitis B virus (HBV) DNA, and several factors related to the selection of primer pairs for the PCR amplification have been demonstrated. The sensitivity of the PCR assay was compared with that of slot-blot hybrid