Analysis of hydroxyproline by high performance liquid chromatography and its application to collagen turnover studies in bone cultures
โ Scribed by Sheila Rydziel; Ernesto Canalis
- Book ID
- 110554628
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 324 KB
- Volume
- 44
- Category
- Article
- ISSN
- 1432-0827
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๐ SIMILAR VOLUMES
Collagen extracted from tissues by pepsin digestion is a mixture of monomeric and oligomeric molecules. The oligomers are held together by covalent crosslinks between molecules. A simple size-exclusion high-performance liquid chromatography (HPLC) method for separation of collagen monomers and oligo
## Abstract A simple, specific and sensitive HPLC method with UV detection was developed and validated for the determination of tectoridin in rat plasma for the first time. Chromatographic separation was performed on a Welchrom^TM^ C~18~ column (150 ร 4.6ย mm, i.d., 5ย ยตm) at a flow rate of 1.0ย mLย mi