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Analysis of hydroxy and keto cholesterols in oxidized brain synaptosomes

✍ Scribed by Govind T. Vatassery; Hung T. Quach; W. Ed Smith; Thomas P. Krick; Frank Ungar


Book ID
107488403
Publisher
Springer-Verlag
Year
1997
Tongue
English
Weight
474 KB
Volume
32
Category
Article
ISSN
0024-4201

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An ultrasensitive HPLC method has been developed for measuring NADP \({ }^{+}\), NADPH, NAD \({ }^{+}\), and NADH. A simple, rapid reaction of the oxidized nucleotides with cyanide in basic solution leads to two stable fluorescent products and allows all four nucleotides to be separated and quantita