✦ LIBER ✦

Analysis of Glycosidic Linkages in Saccharide Compounds by Post-source Decay Fragment Methods in Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectroscopy

✍ Scribed by Tohru Yamagaki; Yasuko Ishizuka; Shin-ichirou Kawabata; Hiroshi Nakanishi

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 177 KB
Volume: 11
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(199703)11:5<527::aid-rcm830>3.0.co;2-h

No coin nor oath required. For personal study only.

✦ Synopsis

Maltotriosyl

-and panosyl-α-cyclodextrins and the nonaose of pullulan were analyzed by post-source decay (PSD) fragment methods of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectroscopy. By the mass number analysis, it was found that all of the PSD fragment ions were produced by cleavages of glycosidic linkages. Comparison of the relative intensities of the ions in those compounds enabled us to distinguish two kinds of glycosidic linkages, α1-4 and α1-6, by MALDI-TOFMS with a new type of ion reflector: the curved field reflectron.

📜 SIMILAR VOLUMES

Accurate peptide sequencing by post-sour

Accurate peptide sequencing by post-source decay matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

✍ Wenjun Mo; Toshifumi Takao; Yasutsugu Shimonishi 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 153 KB 👁 2 views

Partial 18 O-labeling of peptides has been applied to post-source decay experiments in a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer. The ions which originate from the carboxyl terminus of a peptide partially retain 18 O atoms which have readily been incorporated int

Using a matrix-assisted laser desorption

Using a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer for combined in-source decay/post-source decay experiments

✍ Pfeifer, Thomas; Drewello, Mario; Schierhorn, Angelika 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 178 KB 👁 2 views

Mass spectrometric experiments with fragment ions have not yet been possible with a matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer because intense signals of fragment ions were rarely observed during continuous extraction and the mass resolution of in-source

Oligonucleotide Sequencing by Fragmentat

Oligonucleotide Sequencing by Fragmentation in Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

✍ Y.F. Zhu; N.I. Taranenko; S.L. Allman; N.V. Taranenko; S.A. Martin; L.A. Haff; C 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 214 KB 👁 2 views

Oligonucleotide sequencing by fragmentation in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry has been demonstrated. With this sequencing method, the preparation of oligonucleotide ladder products and gel electrophoresis are not required, thus the sequencing

Structural analysis of (methyl-esterifie

Structural analysis of (methyl-esterified) oligogalacturonides using post-source decay matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

✍ Gert-Jan W. M. van Alebeek; Olga Zabotina; Gerrit Beldman; Henk A. Schols; Alpho 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 177 KB 👁 2 views

Influence of different glycosidic linkag

Influence of different glycosidic linkages on relative ion intensities in post-source decay fragmentation of a xyloglucan heptaoligosaccharide using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

✍ Tohru Yamagaki; Yasushi Mitsuishi; Hiroshi Nakanishi 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 169 KB 👁 1 views

Post-source decay fragment analysis using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) has been applied to a highly branched xyloglucan heptasaccharide from tamarind seed. All fragment ions were produced by cleavage of the glycosidic linkages, including

Post-source Decay Fragment Spectra of Cy

Post-source Decay Fragment Spectra of Cyclomalto-octaose and Branched Cyclomalto-hexaose by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

✍ Tohru Yamagaki; Yasuko Ishizuka; Shin-ichirou Kawabata; Hiroshi Nakanishi 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 298 KB 👁 1 views

y-Cyclodextrin, maltosyl-a-cyclodextrin and diglucosyl-a-cyclodextrin were analyzed using matrix-assisted laser desorptiodionization timesf-flight (MALDI-TOF) mass spectrometry. AU of these compounds have the same molecular weight (M.W.=1297.15) and consist of only ~-gIucopyrmose. From a comparison