Analysis of fumonisin B1 in corn by capillary electrophoresis with fluorescence detection of the FMOC derivative

✍ Scribed by Manuel Holcomb; Harold C. Thompson Jr.

• Book ID: 102504472
• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 303 KB
• Volume: 7
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/mcs.1220070503

✦ Synopsis

Abstract

A new method has been developed for the analysis of fumonisin B~1~ in corn using capillary electrophoresis. Fumonisin B~1~ is the major fumonisin metabolite produced by the fungus Fusarium moniliforme and has been implicated in human and animal diseases. Fumonisin B~1~ was extracted from corn with acetonitrile/water (50/50) and cleaned up with a Waters C~18~ Sep‐Pak Vac cartridge. Fumonisin B~1~ was quantitated after elution from the C~18~ Sep‐Pak column using capillary electrophoresis (CE) with a 25 mM sodium borate buffer (pH 9.0) containing 10% acetonitrile and fluorescence detection of the FMOC derivative. The minimum detectable amount in corn was 0.5 ppm. Recovery values in spiked corn averaged over 96% over the range 1–20 ppm. © 1995 John Wiley & Sons, Inc.