✦ LIBER ✦

Analysis of DNA in Single Pollen Cell by Hadamard Transform Microscopic Fluorescence Imaging

✍ Scribed by Tang Hongwu; Chen Guanquan; Zeng Yun'e

Publisher: Elsevier Science
Year: 1999
Tongue: English
Weight: 91 KB
Volume: 61
Category: Article
ISSN: 0026-265X
DOI: 10.1006/mchj.1999.1575

No coin nor oath required. For personal study only.

✦ Synopsis

A noncommercial Hadamard transform microscopic image analysis system was used to measure fluorescence emitted by single cells. A method for subtracting the native fluorescence of the Zephyranthes candida(Lindl.) Herb. pollen cell was developed based on its correlation to pollen area. Acridine orange and ethidium bromide were used as fluorescence probes for pollen DNA quantitative analysis, and the results obtained by the two methods were compared.

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