Analysis of Different Promoter Systems for Efficient Transgene Expression in Mouse Embryonic Stem Cell Lines
✍ Scribed by Sangmi Chung; Therese Andersson; Kai-C. Sonntag; Lars Björklund; Ole Isacson; Kwang-Soo Kim
- Book ID
- 111843328
- Publisher
- AlphaMed Press
- Year
- 2002
- Tongue
- English
- Weight
- 252 KB
- Volume
- 20
- Category
- Article
- ISSN
- 1066-5099
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A tk+/mouse embryonic stem (ES) cell line, designated 1G2, has been created in which one allele of the thymidine kinase (tk) gene was inactivated by targeted homologous recombination. This line is an analog of the mouse lymphoma tk+/-L5 178Y cell line, which is used widely to assess the mutagenicity
Stem cells have the potential to be differentiated to a specific cell type through genetic manipulation and therefore, represent a new and versatile source of cell replacement in regenerative medicine. However, conventional ways of gene transfer to these progenitor cells, suffer from a number of dis