Analysis of Dicamba Degradation by Pseudomonas maltophilia Using High-Performance Capillary Electrophoresis

✍ Scribed by J. Yang; X.Z. Wang; D.S. Hage; P.L. Herman; D.P. Weeks

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 453 KB
• Volume: 219
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1994.1228

✦ Synopsis

A method based on high-performance capillary electrophoresis (HPCE) was developed for the simultaneous analysis of dicamba and its metabolites in media containing the bacterium Pseudomonas maltophilia. Dicamba, 3,6-dichlorosalicylic acid (DCSA), and related products were extracted from media samples using ether under acidic conditions and injected onto an HPCE system containing a pH 10.0 running buffer. Baseline resolution between these compounds was obtained at (30 \mathrm{kV}) with a total run time of (6 \mathrm{~min}) on a 50 (\mu \mathrm{m}) i.d. (\times 50-\mathrm{cm}) capillary. The linear range for dicamba and DCSA detected at (274 \mathrm{~nm}) extended from 0 to 100 (\mathrm{mg} / \mathrm{liter}) and the dynamic range for both compounds extended to (2000 \mathrm{mg} /) liter. The within-run precision was (\pm 5 %) or less throughout the entire concentration range studied. The limits of detection for dicamba and DCSA in the media samples were 6 and (2 \mathrm{mg} / \mathrm{liter}). This corresponded to detection limits of 0.3 and (0.1 \mathrm{ng}), respectively, in the injected extracts. With this method it was possible to conduct preliminary studies examining the kinetics of dicamba metabolism in (P). maltophilia. (c) 1984 Academic Press, Inc.