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Analysis of conifer leaf free amino acids by gas-liquid chromatography

✍ Scribed by S.L. MacKenzie; K.R. Holme

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
602 KB
Volume
299
Category
Article
ISSN
1873-3778

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✦ Synopsis

Free amino acids from the leaves of ten North American conifer species have been analysed by packed-column gas-liquid chromatography as their N(O,S)-heptafluorobutyryl isobutyl esters. The species examined were Balsam fir [A&es balsumea (L.) Mill], douglas fir [Pseudotsuga menzeisii(Mirb.) France], western hemlock [Tsugu heterophyllu (Raf.) Sarg.], Rocky mountain juniper (Juniperus scopulorum Sarg.), western juniper (Juniperus occidentalis, Hook), lodgepole pine (Pinus contorru Dougl. var. latifolia Engelm.), ponderosa pine (Pinus ponderosa Laws.), black spruce [Picea mariunu (Mill.) BSP], Colorado spruce (Piceu pungens Engelm.), and white spruce [Piceu gluucu (Moench) (Voss)]. All the proteic amino acids and some biologically important non-proteic amino acids were readily assayed. Gas chromatography-mass spectrometry was used to confirm the identity of the proteic amino acids and the homogeneity of the corresponding peaks. Ten unidentified components were partially characterised by their mass spectra.

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