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Analysis of coenzyme M (2-mercaptoethanesulfonic acid) derivatives by isotachophoresis

✍ Scribed by J.M.H. Hermans; T.J. Hutten; C. van der Drift; G.D. Vogels

Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 315 KB
Volume: 106
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90533-3

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✦ Synopsis

2-Mercaptoethanesulfonic acid (coenzyme M, HS-CoM), methylcoenzyme M (CHa-S-CoM), acetylcoenzyme M (CH,CO-S-CoM), 2,2'-dithiodiethanesulfonic acid [(S-CoM),], and bromoethanesulfonic acid can be simultaneously and conveniently determined by isotachophoretic analysis. Amounts as low as 10 pmol can be detected. The reproducibility of the method is within 2%. The reduction of (S-CoM)% and the formation of CH,-S-CoM from HS-CoM and methanol by dialyzed cell-free extracts of Methanosarcina barkeri were studied. ' Abbreviations used: HS-CoM, (S-CoM), and CH,-S-CoM, 2-mercaptoethanesulfonic acid (coenzyme M) and its oxidized and methylated form; Tes, N-tris (hydroxymethyl)methyl-2-aminoethanesulfonic acid.

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