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Analysis of cell cultures of Taxus wallichiana using direct analysis in real-time mass spectrometric technique

✍ Scribed by Suchitra Banerjee; K. P. Madhusudanan; Suman P. S. Khanuja; Sunil K. Chattopadhyay


Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
105 KB
Volume
22
Category
Article
ISSN
0269-3879

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✦ Synopsis


Abstract

The applicability of a new mass spectrometric technique, DART (direct analysis in real time) has been studied in the analysis of the calli of Taxus wallichiana. The intact callus samples were directly analyzed by holding them in the gap between the DART source and mass spectrometer for measurements. Five C‐14 oxygenated taxoids were characterized from the analysis of the calli of the Taxus wallichiana almost instantaneously. The confirmation of the structures of the identified taxoids was made through their accurate molecular formula determinations. Copyright © 2007 John Wiley & Sons, Ltd.


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