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Analysis of biological thiols: Derivatization with monobromobimane and separation by reverse-phase high-performance liquid chromatography

✍ Scribed by Gerald L. Newton; Randel Dorian; Robert C. Fahey

Book ID
118838996
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
323 KB
Volume
114
Category
Article
ISSN
0003-2697

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Analysis of biological thiols: Quantitat
Analysis of biological thiols: Quantitative determination of thiols at the picomole level based upon derivatization with monobromobimanes and separation by cation-exchange chromatography
✍ Robert C. Fahey; Gerald L. Newton; Randel Dorian; Edward M. Kosower πŸ“‚ Article πŸ“… 1981 πŸ› Elsevier Science 🌐 English βš– 678 KB

A method for quantitative determination of biological thiols is presented. Thiols are converted to fluorescent derivatives by reaction with monobromobimane or monobromotrimethylammoniobimane. The derivatives are separated by ion-exchange chromatography and detected by fluorometry. Thiols that can be