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Amino acid analysis by reverse-phase high-performance liquid chromatography: Precolumn derivatization with phenylisothiocyanate

✍ Scribed by Robert L. Heinrikson; Stephen C. Meredith


Book ID
107712022
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
789 KB
Volume
136
Category
Article
ISSN
0003-2697

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All 24 dansyl amino acids were separated by reverse-phase high-performance liquid chromatography on Develosil C8-5, using a linear gradient made from Tris-HCl buffer (pH 7.75) and methanol. A linear relationship between the amount of sample and peak area was found over the range of 6 to 300 ng (0.02