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Analysis of an immunodominant epitope of topoisomerase I in patients with systemic sclerosis

✍ Scribed by T. M. Meesters; M. Hoet; F. H. J. Hoogen; R. Verheijen; W. J. Habets; W. J. Venrooij

Book ID
104768212
Publisher
Springer
Year
1992
Tongue
English
Weight
737 KB
Volume
16
Category
Article
ISSN
0301-4851

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✦ Synopsis

In this paper an immunodominant epitope of Topoisomerase I is described. An epitope expression sublibrary was constructed from Topoisomerase I cDNA. The subclones were screened with an antiserum from a patient with systemic sclerosis (SSc). The positive clones defined one immunodominant B cell epitope (epitope III), which was located at the carboxyterminal part of the protein. The epitope, 52 amino acids in length, neither contains the p30gag sequence nor the suggested active site Tyr-723, both presumed antibody recognition sites. More than 70% of our anti-TopoI sera recognize this epitope III, indicating that it is a major recognition site of the anti-TopoI autoantibodies in SSc sera. DNA relaxation experiments show that all sera that recognize epitope III and most sera with antibodies to other epitopes inhibit Topoisomerase I activity.

πŸ“œ SIMILAR VOLUMES

An immunodominant epitope on DNA topoiso
An immunodominant epitope on DNA topoisomerase I is conformational in nature: Heterogeneity in its recognition by systemic sclerosis sera
✍ Masataka Kuwana; Junichi Kaburaki; Thomas A. Medsger Jr.; Timothy M. Wright πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 233 KB

## Objective: To characterize an immunodominant epitope recognized by anti-dna topoisomerase i (topo i) antibody, a major autoantibody in sera of patients with systemic sclerosis (ssc). ## Methods: Topo i fragments were generated as fusion proteins using a bacterial expression system as well as p