Analysis of amino acid–carbohydrate mixtures by anion exchange chromatography and integrated pulsed amperometric detection
✍ Scribed by Petr Jandik; Jun Cheng; Nebojsa Avdalovic
- Book ID
- 116616124
- Publisher
- Elsevier Science
- Year
- 2004
- Tongue
- English
- Weight
- 533 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0165-022X
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C a r b o h y d r a t e s s u c h as s u g a r a l c o h o l s , m o n o s a c c h a r i d e s , d i s a c c h a r i d e s , a n d o t h e r o l i g o s a c c h a r i d e s are s e p a r a t e d as a n i o n s by i o n exchange chromatography w i t h a sodium h y d r o x i d e e l u e n t . Ret e n
The method of anion exchange chromatography followed by pulsed amperometric detection (AE-PAD; Johnson, D. C., and Polta, T. Z. (1986) Chromatogr. Forum 1, 37-44) has been applied to the compositional analysis of glycoconjugates. Using 22 mM NaOH as a column effluent, underivatized fucose, galactosa