Determination of Carbohydrates by Anion Exchange Chromatography with Pulsed Amperometric Detection

✍ Scribed by Rocklin, Roy D.; Pohl, Christopher A.

• Book ID: 118743128
• Publisher: Informa UK (Taylor & Francis)
• Year: 1983
• Tongue: English
• Weight: 504 KB
• Volume: 6
• Category: Article
• ISSN: 0148-3919
• DOI: 10.1080/01483918308064876

✦ Synopsis

C a r b o h y d r a t e s s u c h as s u g a r a l c o h o l s , m o n o s a c c h a r i d e s , d i s a c c h a r i d e s , a n d o t h e r o l i g o s a c c h a r i d e s are s e p a r a t e d as a n i o n s by i o n exchange chromatography w i t h a sodium h y d r o x i d e e l u e n t . Ret e n t i o n t i m e a n d s e l e c t i v i t y a r e c o n t r o l l e d by v a r y i n g e l u e n t s t r e n g t h and column t e m p e r a t u r e . The c a r b o h y d r a t e s are d e t e c t e d by o x i d a t i o n a t a g o l d e l e c t r o d e . A r e p e a t i n g s e q u e n c e o f t h r e e p o t e n t i a l s e l e c t r o c h e m i c a l l y c l e a n s t h e e l e c t r o d e s u r f a c e o f o x i d a t i o n p r o d u c t s and o t h e r i n t e r f e r i n g s p e c i e s . D e t e c t i o n l i m i t s are as low as 30 ppb f o r s u g a r a l c o h o l s and m o n o s a c c h a r i d e s , a n d a b o u t 100 ppb f o r o l i g o s a c c h a r i d e s . O t h e r s p e c i e s c o n t a i n i n g CHOH g r o u p s c a n a l s o be d e t e c t e d , s u c h a s a l c o h o l s and g l y c o l s .