Analysis and Purification of Synthetic O
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Martin Gilar
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Article
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2001
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Elsevier Science
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English
β 113 KB
Native and modified synthetic oligonucleotides were purified by reversed-phase HPLC using volatile ionpairing mobile phases. Purification of 10 -90 nmol of oligonucleotides in a single injection was demonstrated using a 4.6 Ψ 75-mm HPLC column packed with porous 2.5 m C18 sorbent. Separation of targ