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Analysis of 2′,5′-oligoadenylates in cells and tissues

✍ Scribed by Tony R. Reid; Carol Lipsey Hersh; Ian M. Kerr; George R. Stark

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
498 KB
Volume
136
Category
Article
ISSN
0003-2697

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✦ Synopsis

Complex mixtures of 2',5'-oligoadenylates are formed in cells and tissues under several different circumstances, and methods for analyzing such mixtures are reviewed. Separation is achieved by high-performance liquid chromatography and quantitation by competition-binding assays, using three different types of antibodies or a specific binding protein, or by functional assay, using preparations of an endonuclease specifically activated by some of the 2',5'-oligoadenylates. Representative results from three different biological systems are presented. The function of 2',5'-oligoadenylates as activators of intracellular RNA degradation is discussed, along with the possibility that these compounds may serve as signals for other intracellular regulatory processes.

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