𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Analyses of Secondary Structures in DNA by Pyrosequencing

✍ Scribed by Mostafa Ronaghi; Malin Nygren; Joakim Lundeberg; Pål Nyrén

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
136 KB
Volume
267
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A common problem in conventional DNA sequencing is the occurrence of DNA sequence compressions during gel electrophoresis, leading to misreading of the sequence. These compressions are usually due to secondary structures in the DNA fragment. In this study, we present a non-gel-based DNA sequencing technique that facilitates analysis of such DNA regions. A part of the polymorphic pertussis toxin promoter region in five different Bordetella species was successfully resolved by the new technique. The obtained sequence data revealed four related palindromic sequences. The ability of different DNA polymerases to read through such secondary structures is also described.

📜 SIMILAR VOLUMES

Analysis of trematode parasite communiti
Analysis of trematode parasite communities in fish eye lenses by pyrosequencing of naturally pooled DNA
✍ Christian Rellstab; Katja-Riikka Louhi; Anssi Karvonen; Jukka Jokela 📂 Article 📅 2011 🏛 Elsevier Science 🌐 English ⚖ 519 KB

Infections by multiple parasite species are common in nature and have important consequences for between-species interactions and coevolutionary dynamics with the host populations. For example, ecological and evolutionary factors underlying the structure of parasite communities determine the range o

Light-Induced Formation of G-Quadruplex
Light-Induced Formation of G-Quadruplex DNA Secondary Structures
✍ Günter Mayer; Lenz Kröck; Vera Mikat; Marianne Engeser; Alexander Heckel 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 167 KB 👁 1 views

The attachment of photolabile "protecting" groups to mask the activity of biologically active compounds is commonly referred to as "caging". [1] The active molecule can be released by irradiation with the laser of a confocal microscope. The method provides exact control over the location, dose and t