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Analyse génétique de sacR, régulateur en cis de la synthèse de la lévane-saccharase de Bacillus subtilis

✍ Scribed by Steinmetz, M.; Aymerich, S.

Book ID
122508935
Publisher
Elsevier Science
Year
1986
Weight
770 KB
Volume
137
Category
Article
ISSN
0769-2609

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✍ Steinmetz, Michel ;Le Coq, Dominique ;Djemia, Hajer Ben ;Gay, Philippe 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 936 KB

The structural gene sacB encoding B. subtilis levansucrase, a secreted enzyme, expresses in E. coli. E. coli hosts of the sacB gene are poisoned by sucrose. This property allowed a powerful selection of mutants affected in the cloned gene. The plasmidic mutations were readily introduced in the B. su