An ultramicro high-performance liquid chromatographic method for assaying ion-pair species of benactyzine

✍ Scribed by Nesbitt D. Brown; H.Kenneth Sleeman

• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 248 KB
• Volume: 140
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)93595-7

✦ Synopsis

Current methodolo,T for measuring the concentration of benactyzine in various types of therapeutic drugs is based primarily on calorimetric and spectrophotometric methods'-i. In most cases, pre-treatment of the sample is required prior to analysis_ Hence, many cf the procedures used in these methods are long and tedious. The sensitivity is limited. Benactyzine levels of less than 5 ,ZU,O are difficult to measure.

We have recently developed a highly sensitive ion-p&

hkh-nerformance ----. ---F--~~--__-~----liquid chromatographic

(HPLC) method for determining benactyzine in various therapeutic drugs using reversed-phase chromatography.

The method is capable of quantifying levels of the compound as low as 200 ng on column. Analysis time is 6 min per sample. No pre-treatment is required_ As a rather simple and specific method, a high degree of precision and accuracy is possible when utilizing this new procedure.