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An optimized protocol for isolation of soluble proteins from microalgae for two-dimensional gel electrophoresis analysis

โœ Scribed by Sheng-Bing Wang; Qiang Hu; Milton Sommerfeld; Feng Chen


Book ID
111588377
Publisher
Springer
Year
2003
Tongue
English
Weight
396 KB
Volume
15
Category
Article
ISSN
0378-1909

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Capillary electrophoresis/time-of-flight mass spectrometry(CE/TOFMS) has been used for analysis of in-gel digests of protein spots excised from two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-D SDS-PAGE). An off-line purification and preconcentration procedure with a Zip