An Optimized 3D NOESY–HSQC

✍ Scribed by Sekhar Talluri; Gerhard Wagner

• Book ID: 102598765
• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 104 KB
• Volume: 112
• Category: Article
• ISSN: 1064-1866
• DOI: 10.1006/jmrb.1996.0132

✦ Synopsis

Three-dimensional 15 N-dispersed NOESY spectra (1-4) gradient g4 defocuses the water which is transverse while the proton-nitrogen spin pair is in longitudinal two-spin have been an invaluable tool for structural studies of proteins and other macromolecules. They consist of a NOESY pulse order, 2N z H z . The gradient g5 following the 15 N evolution period refocuses the water, and the phase f7 of the following sequence that is followed by a 1 H-15 N HMQC (5) or HSQC