An operational approach to N-Cl-α-amino acids decomposition

✍ Scribed by X. L. Armesto; M. L. Canle; M. Losada; J. A. Santaballa

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 415 KB
• Volume: 25
• Category: Article
• ISSN: 0538-8066
• DOI: 10.1002/kin.550250502

✦ Synopsis

It has been usually thought that the rate of N-C1-a-amino acids decomposition changes and that their coefficient of molar absorptivity falls when pH < 5, which has been explained on the basis of a hypothetical process of protonation at the N-C1-amino group.

We have proved: (i) the absence of protonation at the N-C1-amino group, at least when pH > 2-; (ii) the effect of the method of mixing on the reaction rate; (iii) the absolute necessity of a very careful choice of buffers and soIvents; and (iv) the need to check the accuracy of the mathematical model to describe the chemical processes.