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An NMR probe for the study of aerobic suspensions of cells and organelles

✍ Scribed by R.S. Balaban; D.G. Gadian; G.K. Radda; G.G. Wong

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
421 KB
Volume
116
Category
Article
ISSN
0003-2697

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✦ Synopsis

We describe the construction of an NMR probe and cell chamber with good mixing, pH buffering, and oxygenation characteristics, which can be used for relatively dilute cell and organelle suspensions. The "P NMR spectra of acceptable signal-to-noise ratios are obtained from approximately 200 mg (protein) of tissues, and kinetic studies of mitochondrial oxidative phosphorylation are demonstrated. Representative spectra from rabbit kidney cortical tubules and rabbit kidney cortical mitochondria are presented.

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