✦ LIBER ✦

An Improved Perifusion System for NMR Study of Living Cells

✍ Scribed by Y. Boulanger; P. Vinay; M. T. Phan Viet; R. Guardo; M. Desroches

Publisher: John Wiley and Sons
Year: 1985
Tongue: English
Weight: 311 KB
Volume: 2
Category: Article
ISSN: 0740-3194
DOI: 10.1002/mrm.1910020509

No coin nor oath required. For personal study only.

✦ Synopsis

Isolated cells can be kept in steady-state conditions for very long periods of time when inserted into dialysis microfibers and perifused with a nutrient-containing solution. Using this system, stable "P NMR spectra have been obtained on dog kidney tubules for a period Of 13 h.

📜 SIMILAR VOLUMES

An improved tantalum chamber for prolong

An improved tantalum chamber for prolonged microscopic study of living cells in mammals

✍ Williams, Roy G. ;Roberts, Brooke 📂 Article 📅 1950 🏛 John Wiley and Sons 🌐 English ⚖ 811 KB

An NMR probe for the study of aerobic su

An NMR probe for the study of aerobic suspensions of cells and organelles

✍ R.S. Balaban; D.G. Gadian; G.K. Radda; G.G. Wong 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 421 KB

We describe the construction of an NMR probe and cell chamber with good mixing, pH buffering, and oxygenation characteristics, which can be used for relatively dilute cell and organelle suspensions. The "P NMR spectra of acceptable signal-to-noise ratios are obtained from approximately 200 mg (prote

Fluorine as an NMR probe for structural

Fluorine as an NMR probe for structural studies of chemical and biological systems

✍ Yelena G. Gakh; Andrei A. Gakh; Angela M. Gronenborn 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 137 KB 👁 1 views

Applications of fluorine NMR to probe long-range interactions in rigid organic molecules, namely cubanes, and to study structures and dynamics of proteins and other biological systems, are presented. F NMR parameters, especially long-range coupling constants are shown to reveal intrinsic features o

Microscopy under pressure—An optical cha

Microscopy under pressure—An optical chamber system for fluorescence microscopic analysis of living cells under high hydrostatic pressure

✍ Benjamin Frey; Markus Hartmann; Martin Herrmann; Roland Meyer-Pittroff; Karl Som 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 423 KB

## Abstract High hydrostatic pressure (HHP) becomes more and more interesting for life science research, since it can be employed to inactivate various cells. To directly monitor “cells under pressure,” the development of an optical high‐pressure chamber is required. Therefore, an optical pressure

Plaxyloside from the Marine Sponge Plako

Plaxyloside from the Marine Sponge Plakortis simplex: an Improved Strategy for NMR Structural Studies of Carbohydrate Chains

✍ Valeria Costantino; Ernesto Fattorusso; Concetta Imperatore; Alfonso Mangoni 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 304 KB

Study of a fuel cell network with water

Study of a fuel cell network with water electrolysis for improving partial load efficiency of a residential cogeneration system

✍ S. Obara 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 605 KB