## Abstract The concentration of leucine in the growth medium has been found to influence the expression of the temperature sensitive phenotype of a mutant of Chinese hamster ovary cells with an altered leucyl‐tRNA synthetase. Plating efficiency and growth studies showed that increasing the leucine
An investigation into the potential of a mammalian temperature sensitive leucyl-tRNA synthetase mutant for mutagenesis studies
✍ Scribed by S.J. Molnar; A.M. Rauth
- Book ID
- 113190885
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 869 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0027-5107
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## Abstract Nine spontaneous and seven ethyl methanesulfonate induced revertants of the Chinese hamster ovary cell line mutant (__ts__Hl), which possesses a temperature sensitive leucyl‐tRNA synthetase, were isolated and characterized with respect to growth rate, leucyl‐tRNA synthetase activity and
## Abstract The temperature sensitive leucyl‐tRNA synthetase mutant __ts__Hl and two revertants have been compared to the parental Chinese hamster ovary cells with respect to the effects of amino acid concentrations in the medium on growth. Elevating the leucine concentration 30‐ or 100‐fold allowe