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Effect of leucine on the temperature sensitive phenotype of a mammalian leucyl-tRNA synthetase mutant

✍ Scribed by S. J. Molnar; A. M. Rauth

Publisher
John Wiley and Sons
Year
1975
Tongue
English
Weight
417 KB
Volume
85
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

The concentration of leucine in the growth medium has been found to influence the expression of the temperature sensitive phenotype of a mutant of Chinese hamster ovary cells with an altered leucyl‐tRNA synthetase. Plating efficiency and growth studies showed that increasing the leucine concentration allows cells to survive at normally non‐permissive high temperatures and conversely decreasing the leucine concentration enhances the adverse effects of high temperature. A similar but smaller effect was noted with isoleucine. It is suggested that this observation may form the basis of a rapid test, useful in directing the investigation of the lesion in similar mutants to pathways involving specific amino acids.

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